Charles de Menou d'Aulnay (c. 1604–1650) was a pioneer of European settlement in North America and Governor of Acadia (1635–1650).
D'Aulnay was a member of the French nobility who was at various times a sea captain, a lieutenant in the French navy to his cousin Isaac de Razilly, and Governor of Acadia. He was born at Château de Charnizay, Indre-et-Loire, his father being a high-ranking official for Louis XIII.
Isaac de Razilly, having been selected by the government to restore to France her Acadian possessions, became governor of Acadia in 1632, and d'Aulnay was one of his able assistants, borrowing funds, hiring ships, and recruiting men for the regular ocean crossings to and from France for the Compagnie des Cent-Associés and a private company, Razilly-Condonnier. These companies had divergent interests at times which resulted in costly competition. Razilly brought with him forty families and settled at La Hève (near present-day Lunenburg, Nova Scotia) on the southern coast of the island, dispossessing a Scotchman.
In 1635, Razilly re-established French control of Fort Pentagouet at Majabigwaduce on the Penobscot Bay, which had been given to France in an earlier Treaty with the English. He gave the Plymouth men that had charge of the fort their liberty, but bade them tell their people at the English plantations that he would come the next year and displace them as far south as the 40th degree of north latitude. He then took full possession of the place, and strengthened the defences. Plymouth people manned a vessel and went to Penobscot to drive out the French, whom they found only 18 in number, but strongly intrenched. D'Aulnay permitted them to expend all their ammunition, and then go home.