The Caloosahatchee culture is an archaeological culture on the Gulf coast of Southwest Florida that lasted from about 500 to 1750 CE. Its territory consisted of the coast from Estero Bay to Charlotte Harbor and inland about halfway to Lake Okeechobee, approximately covering what are now Charlotte and Lee counties. At the time of first European contact, the Caloosahatchee culture region formed the core of the Calusa domain.
Some Archaic artifacts have been found in the Caloosahatchee culture region, including one site classified as early Archaic. There is evidence that Charlotte Harbor aquatic resources were being intensively exploited before 3500 BC. Undecorated pottery belonging to the early Glades culture appeared in the region around 500 BC. Pottery distinct from the Glades tradition developed in the Caloosahatchee region around 500 AD, and a complex society with high population densities developed by 800 AD. Later periods in the Caloosahatchee culture are defined by the appearance of pottery from other traditions in the archaeological record.
The coast in the Caloosahatchee culture region is a very rich estuarine environment. An extensive network of bays and sounds are protected behind barrier islands. The Caloosahatchee, Myakka and Peace rivers flow into the estuary. There are extensive areas of mangrove and seagrass in the estuary, resulting in high biological production.