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Bisbenzimide

Bisbenzimide
Bisbenzimide.svg
Names
IUPAC name
2’-(4-Ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-1H,3’H-2,5’-bibenzimidazole
Other names
Hoechst 33342
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.041.523
PubChem CID
Properties
C27H28N6O
Molar mass 452.56 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

Bisbenzimide is an organic compound used as a fluorescent stain for DNA in molecular biology applications. Several bisbenzimides are used and are collectively called Hoechst stains.

Bisbenzimides tend to bind to A-T rich regions of DNA and can decrease its density. Bisbenzimide mixed with DNA samples can then be used to separate DNA according to their AT percentage using a cesium chloride (CsCl) gradient centrifugation.


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Wikipedia

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