Bis-tris propane

Bis-tris propane

Names
Other names 2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxymethyl)propane-1,3-diol]
Identifiers
CAS Number	64431-96-5 Y
3D model (Jmol)	Interactive image
Beilstein Reference	1786109
ChEBI	CHEBI:40947 Y
ChemSpider	111383 Y
DrugBank	DB02676 Y
ECHA InfoCard	100.058.981
EC Number	264-899-3
Gmelin Reference	1734507
MeSH	1,3-bis(tris(hydroxymethyl)methylamino)propane
PubChem CID	125132
InChI InChI=1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2 Y Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N Y
SMILES OCC(CO)(CO)NCCCNC(CO)(CO)CO
Properties
Chemical formula	C11H26N2O6
Molar mass	282.34 g·mol−1
Appearance	White crystals
Melting point	164 to 165 °C (327 to 329 °F; 437 to 438 K)
log P	−2.794
Related compounds
Related compounds	Daminozide Octopine Nopaline Pantetheine
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Y  (what is YN ?)
Infobox references

Bis-tris propane, or 1,3-bis(tris(hydroxymethyl)methylamino)propane, is a chemical substance that is used in buffer solutions. It is a white to off-white crystalline powder that is soluble in water. It has a wide buffering range, from 6 to 9.5 due to its two pK_a values which are close in value. This buffer is primarily used in biochemistry and molecular biology.

A review of DNA polymerase fidelity cites bis-tris propane as a suitable buffer for polymerase chain reaction (PCR). Bis-Tris propane has also been used with HCl buffer for stabilization of farnesyl diphosphate isolated from a strain of Saccharomyces cerevisiae. It has also been used in a study of the effects of buffer identity on electric signals of light-excited bacteriorhodospin. Use of Bis-Tris propane has also been documented in an investigation of the MgATPase activity of the myosin subfragment 1 monomer. The effect of buffer identity on the kinetics of the restriction enzyme EcoRV has been studied in various buffers, including Bis-Tris propane. Bis-Tris propane wide buffering range is also useful for calibration of genetically encoded pH indicators expressed in the cytosol or mitochondria.