The Women's 400 metre Hurdles at the 2000 Summer Olympics as part of the athletics programme were held at Stadium Australia on Sunday 24 September, Monday 25 September and Wednesday 27 September 2000.
The top two runners in each of the initial five heats automatically qualified for the semifinals. The next six fastest runners from across the heats also qualified. Those 16 runners competed in 2 heats in the semifinals, with the top three runners from each heat qualifying for the finals. The next two fastest runners from across the heats also qualified.
World and Olympic records prior to the Games.
All times shown are in seconds.
Semi-Finals- Overall