An American Tail Theatre

This is a list of former Universal Studios Florida attractions.

Since Universal Studios Florida's opening on June 7, 1990, a total of 36 attractions have been retired, and usually replaced or re-themed into new attractions, 17 of them being original attractions.

The first major attraction to be retired from the park was An American Tail Theatre in 1992, due to low attendance capacity. The show was replaced in the same year by Beetlejuice's Rock and Roll Graveyard Revue, and the Fievel's Playland attraction, also based on the film An American Tail, opened in the park's Expo Center area simultaneously, and is now part of Woody Woodpecker's KidZone.

In 1996, the interactive show attraction Murder, She Wrote Mystery Theatre was closed due to the cancellation of the Murder, She Wrote television show on which it was based; also retired around the same time was the next door MCA Recording Studio, also an interactive show. The two attractions were replaced the following year by Hercules and Xena: Wizards of the Screen and Stage 54. The Hercules and Xena attraction was later closed and abandoned in 1999, and Stage 54 followed in 2003, with the latter of the two replaced by "Donkey's Photo Finish", an interactive meet-and-greet.

Two further attractions were retired in November 1996. Ghostbusters Spooktacular closed on November 8 due to the end of a contract, and was replaced in the spring of 1998 by Twister...Ride it Out. The nearby The Screen Test Home Video Adventure followed on November 11, and was replaced in the following year with the Islands of Adventure Preview Center. The preview center itself was closed following the grand opening of Islands of Adventure in 1999, and its former space was used for Universal Express distribution, and is now used as an extended queue area for Revenge of the Mummy: The Ride.

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