The 2nd York Militia were a Provincial Militia Line Infantry Regiment at the time of the War of 1812. They were part of the York Militia, which at that time was three Regiments strong. The 2nd Regiment was recruited arould the present-day Halton and Peel Regions.
At the beginning of the War, two Flank Companies were mobilized to fight alongside the 41st Regiment of Foot. One of these was commanded by Captain John Chisholm. They were present at the battle of Queenston Heights, where they heard the (alleged) last words of Major General Isaac Brock, "Push on the brave York volunteers."
The 2nd Regiment remained in the York Militia until sub-units of it broke off to form the Halton Militia in 1846 and the Peel Militia in 1852. These Militia units were broken into Independent Companies in 1856, and were later formed into the 20th Halton Battalion of Infantry and the 36th Peel Battalion in 1866. These two Regiments would later form The Lorne Scots.
The 2nd Regiment of York Militia is perpetuated in the Canadian Army by the Royal Hamilton Light Infantry.