This is a list of players who graduated from the Challenge Tour in 2016. The top 16 players on the Challenge Tour rankings in 2016 earned European Tour cards for 2017.
* European Tour rookie in 2017
Ritthammer won three times on the Challenge Tour in 2016. A change before the season to the tour regulations allowed amateurs to earn ranking points, while also dictating that any player within the top 15 who earned points as an amateur would be counted in addition to the usual 15 graduates; therefore, since Langasque had earned 24,200 points as an amateur, the number of graduates was increased to 16.