The 2010 NCAA Division 1 Softball Tournament was held from May 20 through June 8, 2010 and is part of the 2010 NCAA Division 1 softball season. The 64 NCAA Division 1 college softball teams were selected out of an eligible 284 teams on May 16, 2010. 30 teams were awarded an automatic bid as champions of their conference, and 34 teams were selected at-large by the NCAA Division 1 Softball Selection Committee. The tournament will culminate with 8 teams playing in the 2010 Women's College World Series at ASA Hall of Fame Stadium in Oklahoma City, Oklahoma.
Teams in "italics" advanced to Super Regionals. Teams in "bold" advanced to Women's College World Series.