The 2009–10 Libyan Cup was the 20th edition of the competition since its inception in 1976. Nasr secured their third title, defeating Madina 2–1 in a close final at the 11 June Stadium.
The draw for the Round of 32 was made on January 7, 2010. If scores are level after 90 minutes, then the tie goes to a penalty shootout. Ties were played from January 18–20.
1 Score after 90 minutes
The draw for the round of 16 was made on January 28, 2010 at 21:30 EET.
1 Score after 90 minutes
1 Madina awarded tie, after Ahly Tripoli quit the competition amid protests with the Libyan Football Federation.
Draw made on May 20, at LFF headquarters in Tripoli.