This is a list of division winners and playoff matches in the regionally-organized Eccellenza 2009–2010, which is the 6th level of Italian football.
1.^ Montesilvano deducted one point.
Semi-finals
Final
Mosciano qualified to the National Play-off.
San Nicola Sulmona and Penne relegated to the Promozione.
1.^ Ferrandina and Irsinese deducted one point.
Semi-finals
Final
Angelo Cristofaro qualified to the National Play-off.
Final
Banzi relegated to the Promozione.
Semi-finals
Final
Scalea qualified to the National Play-off.
Città di Amantea relegated to the Promozione.
Semi-final
Final
Arzanese qualified to the National Play-off.
Parete relegated to the Promozione.
Final
Ebolitana qualified to the National Play-off.
Baia 2006 and Striano relegated to the Promozione.
Bagnolese promoted to the Serie D while Virtus Pavullese qualified to the National Play-off.
Meletolese and Reno Centese relegated to the Promozione.
Stuoie Baracca Lugo and Comacchio Lidi relegated to the Promozione.
Rivignano and Sevegliano relegated to the Promozione.
Fidene promoted to the Serie D while Anziolavinio qualified to the National Play-off.
Monterosi relegated to the Promozione.
Colleferro and Torrenova relegated to the Promozione.
Semi-final
Final