The 2001 Exxon Superflo 12 Hours of Sebring was the 49th running of this event. It was both the second round of the 2001 American Le Mans Series season and the opening round of the new European Le Mans Series. It took place at Sebring International Raceway, Florida, on March 17, 2001.
French Porsche driver and ambassador Bob Wollek was killed nearby the circuit following a practice session. Wollek had been riding a bicycle along a nearby highway when he was struck by a van. A minute of silence was held prior to the start of the race. Wollek's team, Petersen Motorsports, withdrew from the event out of respect.
Class winners in bold.
† - #47 Broadfoot Racing was disqualified during the race for making an illegal repair to their car. #34 Orbit was disqualified during the race for receiving outside assistance while still on the track.