The 2000 All-Big Ten Conference football team consists of American football players chosen as All-Big Ten Conference players for the 2000 NCAA Division I-A football season. The conference recognizes two official All-Big Ten selectors: (1) the Big Ten conference coaches selected separate offensive and defensive units and named first- and second-team players (the "Coaches" team); and (2) a panel of sports writers and broadcasters covering the Big Ten also selected offensive and defensive units and named first- and second-team players (the "Media" team).
Bold = selected as a first-team player by both the coaches and media panel
Coaches = selected by Big Ten Conference coaches
Media = selected by a media panel