| Season | 2000–01 |
|---|---|
| Champions | Hamilton Academical |
| Promoted |
Hamilton Academical Cowdenbeath |
| Matches played | 180 |
|
← 1999–00
2001–02 →
|
|
The 2000–01 Scottish Third Division was won by Hamilton Academical who, along with second placed Cowdenbeath, gained promotion to the Second Division. Elgin City finished bottom.