The 1989–90 Southern Football League season saw Dover Athletic win the Premier Division. However Dover cide were unable to get a promotion due to ground grading issues and a place in Football Conference was taken by Bath City. 4 bottom teams were relegated to the Southern and Midland divisions, while champions and runners-up of lower divisions get a places in the next Premier Division season.
The Premier Division featured five new clubs:
The Midland Division featured five new clubs:
The Southern Division featured four new clubs: