The 1960–61 Segunda División season saw 32 teams participate in the second flight Spanish league. Osasuna and Tenerife were promoted to Primera División. Barakaldo, Terrassa, Extremadura, Rayo Vallecano, Sestao and Castellón were relegated to Tercera División.
1Condal renounce to Segunda División after the season ended. Sporting won a promotion play off and stayed in Segunda División.