Surround Optical Fiber Immunoassay

Surround optical fiber immunoassay

A prototype version of surround optical fiber immunoassay
Acronym	SOFIA
Uses	Medical, food safety, industrial, veterinary
Notable experiments	Detection of prions in urine and blood of preclinical carriers
Inventor	Los Alamos National Laboratory and SUNY
Model	Prototype

Surround optical fiber immunoassay (SOFIA) is an ultrasensitive, in vitro diagnostic platform incorporating a surround optical fiber assembly that captures fluorescence emissions from an entire sample. The technology's defining characteristics are its extremely high limit of detection, sensitivity, and dynamic range. SOFIA’s sensitivity is measured at the attogram level (10⁻¹⁸g), making it about one billion times more sensitive than conventional diagnostic techniques. Based on its enhanced dynamic range, SOFIA is able to discriminate levels of analyte in a sample over 10 orders of magnitude, facilitating accurate titering.

As a diagnostic platform, SOFIA has a broad range of applications. Several studies have already demonstrated SOFIA’s unprecedented ability to detect naturally occurring prions in the blood and urine of disease carriers. This is expected to lead to the first reliable ante mortem screening test for vCJD, BSE, scrapie, CWD, and other transmissible spongiform encephalopathies. Given the technology’s extreme sensitivity, additional unique applications are anticipated, including in vitro tests for other neurodegenerative diseases, such as Alzheimer's and Parkinson's.

SOFIA was developed as a result of a joint-collaborative research project between Los Alamos National Laboratory and State University of New York, and was supported by the Department of Defense's National Prion Research Program.